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1.10 - 1.13 Chromatography
Chromatography is another important separation technique. Several different types of chromatography exist:
paper chromatography, thin layer chromatography, gas chromatography
1.11 Activity 1. Separating spinach
1.11 understand how a chromatogram provides information about the composition of a mixture
1.12 understand how to use the calculation of Rf values to identify the components of a mixture
1.13 practical: investigate paper chromatography using inks/food colourings
Watch the video "separating spinach"and then the animation "identity check".
1.11 Activity 2. Running a chromatogram
Watch the video and work through the animation and then answer the questions below.
1.11 Activity 2. Identity check
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Rf stands for retention factor. It gives a measure of how fast a given substance passes through the chromatogram.
The Rf value of a given substance can help to identify it.
Substances which dissolve better in the mobile phase will travel faster and have a higher retention factor.
A different method
This method uses sticks of chalk as the stationary phase and an alcohol/ water mixture as the mobile phase. As with all chromatography the different components are separated because certain components in the mixture are more soluble than others. Watch the video and answer the questions.
- What does this procedure show about the composition of the various inks used?
- Why would it not be easy to obtain accurate Rf values using a method like this?
- What is the solvent ( mobile phase ) used in this method?